How to make your own oyster mushroom substrate?

Table of Contents:

1. Oyster mushroom substrate recipe
2. What substrate is suitable for mushrooms?
3. How to make a substrate easily?
4. What else is important to know?
5. Why do spots appear in the substrate?

Oyster mushroom substrate recipe

YouTube says it's simple:

1. pour boiling water over the components that were at hand (leaves, cardboard, shavings, sawdust),
2. held for 5-8-12 hours (each mushroom grower recommends different numbers),
3. you can make mushroom bags.

On the picture: an example of how NOT to do steaming.

What substrate is suitable for mushrooms?

If you are growing a few bags for yourself, you can fill the raw materials with a lime solution.

You can use this method as long as the mycelium grows well in the substrate.

If black or green mold is constantly appearing in the substrate, you can use the method described below.

I'll first describe a mushroom substrate recipe using sunflower husks and straw, but if you don't have access to those materials, below are some other mushroom substrate materials.

Diy mushroom substrate

The best result at home gives a substrate of sunflower husks.

It has much less mold spores than straw.

Therefore, it takes only 3 hours to heat the husk. And straw - at least 6 hours.

The yield of mushrooms on the husk is higher than on straw.

Be sure to add legume grass hay to the straw to increase the yield.

If you watched a video on YouTube and want to make a substrate from sawdust, foliage, paper, make small portions.

Can you use just coco coir for mushroom substrate? How do you add coffee to mushroom substrate? Is it possible to add sawdust? Yes, you can try any raw material.

In principle, wood-decaying fungi can grow on any raw material that contains cellulose and lignin.

How to make a substrate easily?

First, you need to find nitrogen content charts for the materials you plan to use. The substrate for oyster mushrooms should contain no more than 0.8 units of nitrogen, according to Kjeldahl. If you find a table where crude protein is indicated, then its content in the substrate should be no more than 5.

If the materials you want to use contain more protein, you need to mix the two types of raw materials. For example, any straw with a small amount of protein plus raw materials with a large amount of nitrogen.

How to conduct experiments?

1. Make small portions of the substrate, varying the time the raw materials are in hot water. The water temperature cannot be changed!
2. Do not mix more than two types of raw materials. Make one base, such as any straw. Divide it into several parts. To one part add one type of other raw material that contains a lot of nitrogen (for example, hay), to the other part - another type (for example, cotton tow or cocoa shell).
3. Bran and grain flour have good nutritional value, but they cannot be used using the hot water method, since mold grows in the substrate. These additives are used in the method of sterilizing raw materials in an autoclave.
4. Sawdust from leaf trees has a low nitrogen content and absorbs a lot of water, which is why bacteria multiply there. But the oyster mushroom mycelium does not grow. Therefore, if you want to try using sawdust, experiment with a small amount. Sawdust is used as a basis for the sterilization method in combination with raw materials that have a lot of nitrogen.

Homemade mushroom substrate

Which substrate is better - from straw or sunflower husks

At home, it is easier to grow on the husk:

• The husk contains less spores, mold and bacteria, so its heat treatment takes 3-4 hours, and the straw is processed 7-8 hours.
• The husk is easy to pack up in bags.
• Primordia are formed 2-4 days earlier on sunflower husks, its nutritional value is higher than in straw.
• Straw needs to be chopped on a straw cutter and hay should be added to it (so that the yield is higher), but the husk is ready for use.

Why do we fill raw materials with hot water?

We need to destroy the competitors of oyster mushroom mycelium: mold spores and bacteria.

It is impossible to heat raw materials during heat treatment above 75C.

At the same time, some of the spores not only survive, but also receive thermal activation - after cooling, they swell and grow faster and more aggressively than the rest of the spores.

How many raw materials do you need to take for one mushroom block?

From one kilogram of dry sunflower husk, straw, hay, you can get from 2.5 to 3 kilograms of finished steamed mass.

In other words, to make 10 mushroom blocks weighing 10 kg each, you need 25 to 30 kg of dry straw (or its mixture with hay) or husks. Plus three kilograms of grain spawn (if you add 3%)

What else is important to know?

1. If you didn’t have enough grain spawn, or the bags ran out, and the steamed raw materials are still left, you will have to throw it away. You won't be able to use it again!
2.  The time required for heat treatment does not depend on the volume of the container.
3.  The tank, barrel or bath in which heat treatment is carried out must be completely (the lid too) pasted over with foam plastic or foil glass wool.
   If the container is not insulated, then near the walls the raw material will cool faster, part of it will not warm up, and the likelihood of damage will increase dramatically.

   If you steam raw materials in a two-hundred-liter barrel, you can make 5, maximum 6 mushroom blocks weighing 8.5-9 kg.

5. If you have just started growing oyster mushrooms, you can steam the raw materials by pouring hot water over them.
In doing so, be sure to:

• water must be heated to such a temperature that after you pour it into the raw material, the temperature of the raw material is at least 73-75 degrees;
• the container must be insulated. This is necessary for high-quality heating of the entire volume of the husk and so that the temperature does not drop during heat treatment;
• keep the sunflower husk at this temperature for at least 4 hours.

  If your raw material is straw, I do not recommend this method at all.

• Immediately after the raw material has been steamed, the empty barrel (container) must be washed with clean water and treated with a solution containing sodium hypochlorite. For example, Domestos. Dilute in water as instructed.

But this method is possible ONLY if there is a special room for inoculating mycelium (grain spawn) into the substrate - a clean zone or also called a sowing zone. It must be constantly treated to kill mold spores and bacteria.

Unfortunately, even the presence of a clean zone does not guarantee that by pouring raw materials with hot water, you can avoid the appearance of green mold, non-growth spots, and bacterial infection.

If this happens, you will have to switch to hydrothermia*.

You see in the photo - inoculation takes place outdoors
Heat-treated straw is not even covered.

There will definitely be problems in a few months of such work.

Why do spots appear in the substrate?

Mold and bacteria spores are covered with a thick shell and die at a temperature of at least 121 degrees for 40 minutes.
Therefore, when pouring straw or husks with boiling water (or hot 85-90 degree water), we get rid of only those spores that have swollen or hatched from the shell at the time of heating.
The rest of the spores can germinate simultaneously with the mycelium, and make it a significant competitor.

The longer you grow oyster mushrooms, the more mold and bacteria spores accumulate.

There remain spores that are more resistant to heat treatment.

In the room where the inoculation takes place, spores are most dangerous. Due to high humidity, their number increases.
When germinating, the hyphae of both spores and oyster mushroom mycelium secrete certain substances that inhibit the growth of competitors. It is not always possible for oyster mushroom mycelium to win this fight.

pH in the substrate should be in the range of 7.8-8.2

If there are no problems with overgrowing of the substrate, most likely the pH is in order.
But, when green mold (Trichoderma) appears in the bags, it is necessary to buy a pH meter and measure the alkalinity of the substrate before stuffing.
Read more about pH-substrate here.

* An article about the hydrothermal method